Androgen Receptor Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB66147-50, MB66147-100
Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Swiss-Prot: P10275
Host: Mouse
Reactivity: Human
Applications: WB, IHC
All Applications: WB (1/500 - 1/1000), IHC (1/100 - 1/300)
Background: Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding . This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of Androgen Receptor protein.
Bioworld Molecular Weight: ~ 115 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Androgen Receptor expression in LNCaP (A) whole cell lysates., Immunohistochemical analysis of Androgen Receptor staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Androgen Receptor staining in human prostatic carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Alternative Name: DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Androgen Receptor. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified