ANGPT4 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS60368-50, BS60368-100
Sizes: 50µl, 100µl
Swiss-Prot: Q9Y264
Host: Rabbit
Reactivity: Human
Applications: WB, IHC
Background: Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. Ang-1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), is a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression is restricted to sites of vascular remodeling. Ang-3 and Ang-4 represent the mouse and human counterparts of the same gene locus. The structural divergence of Ang-3 and Ang-4 cause their divergent functions. Ang-3 and Ang-4 have very different distributions in their respective species, and Ang-3 appears to act as an antagonist while Ang-4 appears to function as an agonist. Ang-3 and Ang-4 share all the main structural characteristics of Ang-1 and Ang-2 and are homologous throughout the signal peptide, N-terminal region, coiled-coil segment and Fibrinogen-like domain.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ANGPT4 polyclonal antibody detects endogenous levels of ANGPT4 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ANGPT4 pAb at 1:500 dilution
Lane 1: HCC827 whole cell lysate (40ug)
Lane 2: H460 whole cell lysate (40ug)
Lane 3: H1792 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ANGPT4 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing secreted staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 56 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Angiopoietin-4; ANG-4; Angiopoietin-3; ANG-3; Angiopoietin4; ANG4; Angiopoietin3; ANG3; ANG3; ANG4
Immunogen: A synthetic peptide corresponding to residues in Human ANGPT4.
Conjugate: Unconjugated
Modification: Unmodified