Product Description

ANXA7 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS60862-50, BS60862-100

Sizes: 50µl, 100µl

Swiss-Prot: P20073

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain, which binds to phospholipids in a Ca++-dependent manner, and a unique amino terminal region, which may confer binding specificity. The annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype. It has been hypothesized that growth-dependent post-translational modifications of Annexins are required for proper sub-cellular localization Annexin VII, also referred to as synexin, is located at the plasma membrane in normal muscle tissue. However, in muscle samples from patients suffering from Duchenne’s muscular dystrophy, Annexin VII, along with Annexins IV and VI, is released into the cytoplasm and later, as the disease progresses, into the extracellular space. Two forms of Annexin XI, designated A and B, have been identified. Transfection of COS-7 cells with Annexin XI-A, but not Annexin XI-B, causes formation of Annexin XI-associated vesicles.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ANXA7 polyclonal antibody detects endogenous levels of ANXA7 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ANXA7 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: sp2/0 whole cell lysate, Immunohistochemistry (IHC) analyzes of ANXA7 pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasm,nuclear and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 53 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Annexin A7; Annexin VII; Annexin-7; Synexin; ANXA7; ANX7; SNX

Immunogen: A synthetic peptide corresponding to residues in Human ANXA7.

Conjugate: Unconjugated

Modification: Unmodified