Product Description

AP2M1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS7357-50, BS7357-100

Swiss-Prot: Q96CW1

Host: Rabbit

AP2M1 Polyclonal Antibody-BS7357

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200

Background: The AP-2 coat assembly protein complex is an important component of clathrin-coated pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of α, β, μ, and σ protein subunits. The 50 kDa μ subunit (AP-2μ, AP2M1) is located at the core of the AP-2 complex and mediates interaction between the cargo protein and the clathrin-coated pit (1-4). The carboxy-terminal AP2M1 region recognizes the tyrosine-based, endocytotic sorting motif YXXφ found in cargo proteins and helps to bring the cargo protein to the clathrin-coated pit. Non-canonical, tyrosine-based endocytotic sorting signals can also promote interaction between cargo proteins and AP2M1 (5,6). AP2M1 plays an essential role in molecular signaling as it couples receptor-mediated endocytosis and pathways involving membrane receptors (7-9), matrix metalloproteinases (10), and ion channel proteins (11). Phosphorylation of specific AP2M1 residues and binding of lipids to this adaptor protein can regulate AP2M1 activity (12,13). Phosphorylation of AP2M1 at Thr156 by adaptor-associated kinase 1 (AAK1) stimulates affinity binding of AP2M1 to cargo protein signals (14).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AP2M1 polyclonal antibody detects endogenous levels of AP2M1 protein.

Bioworld Molecular Weight: ~49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of AP2M1 polyclonal antibody, Immunohistochemistry (IHC) analysis of AP2M1 polyclonal antibody in paraffin-embedded Rat Kidney tissue.

Alternative Name: Adapter-related protein complex 2 mu subunit;
Adaptin mu 1;
Adaptin-mu2;
Adaptor protein complex AP 2 subunit mu;
Adaptor protein complex AP-2 subunit mu;
Adaptor related protein complex 2 mu 1 subunit;
AP 2 mu 2 chain;
AP-2 complex subunit mu;
AP-2 mu chain;
AP2M 1;
Ap2m1;
AP2M1_HUMAN;
AP50;
CLAPM1;
Clathrin adaptor complex AP2 mu subunit;
Clathrin assembly protein complex 2 medium chain;
Clathrin associated/assembly/adaptor protein medium 1;
Clathrin coat adaptor protein AP50;
Clathrin coat assembly protein AP50;
Clathrin coat associated protein AP50;
Clathrin coat-associated protein AP50;
HA2 50 kDA subunit;
mu2;
Plasma membrane adaptor AP 2 50kDA protein;
Plasma membrane adaptor AP-2 50 kDa protein;

Immunogen: Recombinant full length Human AP2M1.

Modification: Unmodified

Conjugate: Unconjugated