Apaf-1 Polyclonal Antibody-BS90065

Apaf-1 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS90065-50, BS90065-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: O14727(Human) O88879(Mouse) Host: Rabbit Reactivity: Human, Mouse Applications: WB, ICC/IF, IHC All Applications: WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 Background: The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg²⁺-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFkB in response to bacterial lipopoly-saccharides. Purification and Purity: ProA affinity purified Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity: Apaf-1 polyclonal antibody detects endogenous levels of Apaf-1 protein. Bioworld Molecular Weight: 141 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of Apaf-1 on HUVEC cell lysates using anti-Apaf-1 antibody at 1/1,000 dilution., ICC staining Apaf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alternative Name: APAF-1, APAF1, APAF 1, Apaf-1, APAF, Apaf1, Apoptotic peptidase activating factor 1, Apoptotic protease activating factor 1, Apoptotic protease activating factor, Apoptotic protease-activating factor 1, CED 4, CED4, KIAA0413, Immunogen: recombinant protein Conjugate: Unconjugated Modification: Unmodified