Apaf-1 Polyclonal Antibody - BT-AP00501

Apaf-1 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP00501-20, BT-AP00501-50, BT-AP00501-100

Citations, Manuals and MSDS Available upon request.

Background: APAF1 encodes a cytoplasmic protein that initiates apoptosis. Apoptotic protease-activating factor 1 contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Research Areas: Cell Biology; Cancer

Target Protein: APAF1

Applications: WB, IHC-p, ELISA

Reactivity: Human, Mouse, Rat

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: Apaf-1 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 141840

UniProt Accession: Human: O14727; Mouse: O88879; Rat: Q9EPV5

Synonyms: APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1

GeneID: Human: 317; Mouse: 11783; Rat: 78963

Research Use Only