Product Description

APAF1 Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS40093-50, BS40093-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: APAF Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Induced by E2F and p53 in apoptotic neurons. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. 6 isoforms of the human protein are produced by alternative splicing.

Category: Primary Antibody

Reactivity: Human Mouse

Host: Rabbit

Applications: WB, IHC, ICC/IF

Alternate Names: APAF, APAF-1, APAF1, apoptotic peptidase activating factor 1, apoptotic protease activating factor 1, Apoptotic protease-activating factor 1, CED4, DKFZp781B1145, KIAA0413

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: A synthesized peptide derived from human APAF1

Dilution: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

Purification: Affinity-chromatography

Specificity: APAF1 Antibody detects endogenous levels of total APAF1

Molecular Weight: ~ 130 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O14727

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only