Product Description

APC6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90068-50, BS90068-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13042(Human) Q8R349(Mouse) Unigene:66098(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: APC6 polyclonal antibody detects endogenous levels of APC6 protein.

Bioworld Molecular Weight: 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution., ICC staining APC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: APC6, CDC16Hs, CDC16, ANAPC6, Anaphase promoting complex subunit 6, Anaphase-promoting complex subunit 6, Apc 6, APC6, CDC 16, CDC16 (cell division cycle 16 S. cerevisiae homolog), Cdc16, CDC16 homolog, CDC16 protein, CDC16, CDC16Hs, Cell division cycle 16, Cell division cycle 16 homolog, Cell division cycle protein 16 homolog, CUT9, Cyclosome subunit 6,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified