APE1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10554-50, MB10554-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P27695
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-F, IHC-P, ICC/IF
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Background: Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway. Repair activities of Ape1 are stimulated by interaction with XRCC1, another essential protein in BER. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 36 kDa; Observed MW: 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of APE1 in K562, C6, 3T3, Hela lysates using APE1 antibody., Immunocytochemistry analysis of APE1 in Hela using APE1 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human tonsil using APE1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: APEX1; APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1
Immunogen: Recombinant protein of human APE1
Conjugate: Unconjugated
Modification: Unmodified