APE1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9065-50, MB9065-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P27695 Human;P28352 Mouse
Host: Mouse
Reactivity: Human, Mouse
Applications: WB, IHC FC
All Applications: WB:1:1,000-1:5,000
IHC:1:50-1:200
FC:1:50-1:100
Background: The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: APE1 monoclonal antibody detects endogenous levels of APE1 protein.
Bioworld Molecular Weight: 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of APE1 on HL-60 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature., Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APE1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-13 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Alternative Name: APEN, AP endonuclease 1, APE-1, APEX1, APE, APE1, APEX, APX, HAP1, REF1, AP endonuclease 1, AP endonuclease class I, AP lyase, APE 1, APE, APE-1, APEN, APEX 1, APEX, APEX nuclease (multifunctional DNA repair enzyme) 1, Apex nuclease 1, APEX nuclease, APEX1, APEX1, Apurinic endonuclease, Apurinic-apyrimidinic endonuclease 1, Apurinic/apyrimidinic (abasic) endonuclease, Apurinic/apyrimidinic endonuclease 1, Apurinic/apyrimidinic exonuclease, APX, BAP1, Deoxyribonuclease (apurinic or apyrimidinic), DNA (apurinic or apyrimidinic site) lyase, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, EC 4.2.99.18, HAP 1, HAP1, Human Apurinic endonuclease 1, MGC139790, Multifunctional DNA repair enzyme, Redox factor 1, Redox factor-1, REF 1, REF 1 protein, REF-1, REF1, REF1 protein antibody
Immunogen: Recombinant protein within Human APE1 aa 20-350.
Conjugate: Unconjugated
Modification: Unmodified