APEX1 (R221) Polyclonal Antibody-BS1971

APEX1 (R221) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1971-50, BS1971-100

Sizes: 50µl, 100µl

Swiss-Prot: P27695

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: APEX1 (R221) polyclonal antibody detects endogenous levels of APEX1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of APEX1/Ref-1 (R221) pAb at 1:500 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: MEF whole cell lysate (40ug)
Lane 4: C6 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of APEX1 (R221) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 37 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: AP lyase; APE 1; APE; APE-1; APEN; APEX 1; APEX; APEX nuclease (multifunctional DNA repair enzyme) 1; Apex nuclease 1; Apex nuclease; APEX1; Apurinic endonuclease; Apurinic-apyrimidinic endonuclease 1; Apurinic/apyrimidinic (abasic) endonuclease; Apurinic/apyrimidinic endonuclease 1; Apurinic/apyrimidinic exonuclease; APX; BAP1; Deoxyribonuclease (apurinic or apyrimidinic); DNA (apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; mit

Immunogen: Synthetic peptide, corresponding to amino acids 190-240 of Human APEX1.

Conjugate: Unconjugated

Modification: Unmodified