Product Description

APEX1 (R221) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1971-50, BS1971-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The role of transcription factors in the regμlation of gene expression is well established. Although the activity of these factors can be regμlated by phosphorylation, evidence has indicated regμlation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regμlation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellμlar redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: AP lyase, APE 1, APE, APE-1, APEN, APEX 1, APEX, APEX nuclease (mμltifunctional DNA repair enzyme) 1, Apex nuclease 1, Apex nuclease, APEX1, Apurinic endonuclease, Apurinic-apyrimidinic endonuclease 1, Apurinic/apyrimidinic (abasic) endonuclease, Apurinic/apyrimidinic endonuclease 1, Apurinic/apyrimidinic exonuclease, APX, BAP1, Deoxyribonuclease (apurinic or apyrimidinic), DNA (apurinic or apyrimidinic site) lyase, DNA-(apurinic or apyrimidinic site) lyase, mit

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 190-240 of Human APEX1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: APEX1 (R221) polyclonal antibody detects endogenous levels of APEX1 protein.

Molecular Weight: ~ 37 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27695

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only