Product Description

Apg3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90071-50, BS90071-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NT62(Human) Q9CPX6(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200

Background: Atg3 (autophagy-related protein 3), also known as APG3-like, hAPG3 or PC3-96, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. Atg3 catalyzes the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, a reaction that is essential for autophagy (a cellular process that allows for the degradation of organelles and bulk cellular proteins). The process of forming the Atg8-PE conjugate begins with the removal of the C-terminal arginine residue of Atg8 by Atg4, a cysteine protease. The, now exposed, glycine residue is then activated by Atg7 and is then transferred to Atg3 for the final conjugation to PE. This last step can be accelerated by the presence of the Atg12-Atg5 conjugate which functions similarly to an E3 enzyme.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Apg3 polyclonal antibody detects endogenous levels of Apg3 protein.

Bioworld Molecular Weight: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Apg3 on different lysates using anti-Apg3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: K562
Lane 3: HL-60
Lane 4: Hela
Lane 5: Jurkat, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg3 antibody. Counter stained with hematoxylin.

Alternative Name: APG3-like, hApg3, ATG3, APG3, APG3L, 2610016C12Rik, Apg 3, APG3, APG3 autophagy 3 like, APG3 like, APG3, S. cerevisiae, homolog of, APG3-like, APG3L, Apg3p, ATG 3, ATG3, ATG3 autophagy related 3 homolog, ATG3 autophagy related 3 homolog (S. cerevisiae), ATG3, Autophagy 3, S. cerevisiae, homolog of, Autophagy Apg3p/Aut1p like, autophagy related 3, Autophagy related protein 3, Autophagy-related protein 3, DKFZp564M1178, FLJ22125, hApg3, MGC15201, OTTHUMP00000214547, OTTHUMP00000214548, PC3 96, Protein PC3-96, Ubiquitin-like-conjugating enzyme ATG3,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified