Apg7 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90072-50, BS90072-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O95352(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Apg7 polyclonal antibody detects endogenous levels of Apg7 protein.
Bioworld Molecular Weight: 78 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Apg7 on Jurkat cells lysates using anti-Apg7 antibody at 1/1,000 dilution., ICC staining Apg7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: APG7-like, hAGP7, ATG7, 1810013K23Rik, Apg 7, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), APG7 like, APG7, S. cerevisiae, homolog of, APG7-like, APG7L, ATG 7, ATG12-activating enzyme E1 ATG7, ATG7, ATG7 autophagy related 7 homolog (S. cerevisiae), ATG7 autophagy related 7 homolog, ATG7, Atg7l, Autophagy 7, S. cerevisiae, homolog of, Autophagy related protein 7, Autophagy-related 7 (yeast), Autophagy-related protein 7, DKFZp434N0735, GSA 7, GSA7, hAGP7, Ubiquitin activating enzyme E1 like protein, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-like modifier-activating enzyme ATG7,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified