Product Description

APLF(Phospho-Ser116)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65136-50, BS65136-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q8IW19

Host: Rabbit

Reactivity: Human, Mouse

Applications: IHC-p, IF (paraffin section), ELISA

All Applications: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Background: aprataxin and PNKP like factor(APLF) Homo sapiens C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM, Mar 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-APLF (S116) Polyclonal Antibody detects endogenous levels of APLF protein only when phosphorylated at S116.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Alternative Name: APLF; C2orf13; PALF; XIP1; Aprataxin and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; XRCC1-interacting protein 1

Immunogen: The antiserum was produced against synthesized peptide derived from human APLF around the phosphorylation site of Ser116. AA range:82-131

Conjugate: Unconjugated

Modification: Phosphorylation