Product Description

APOBEC4 Polyclonal Antibody

Catalogue Numbers: BS62444-50, BS62444-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q8WW27

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB:1:500~1:1000

Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system is an adaptive immune response defense mechanism used by archea and bacteria for the degradation of foreign genetic material. This mechanism can be repurposed for other functions, including genomic engineering for mammalian systems, such as gene knockout (KO) and gene activation. CRISPR Activation Plasmid products enable the identification and upregulation of specific genes by utilizing a D10A and N863A deactivated Cas9 (dCas9) nuclease fused to a VP64 activation domain, in conjunction with sgRNA (MS2), a target-specific sgRNA engineered to bind the MS2-P65-HSF1 fusion protein. This synergistic activation mediator (SAM) transcription activation system provides a robust system to maximize the activation of endogenous gene expression.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Specificity: APOBEC4 polyclonal antibody detects endogenous levels of APOBEC4 protein.

Extra Notes: Western blot (WB) analysis of APOBEC4 polyclonal antibody at 1:500 dilution
LaneA:The testis tissue lysate of Rat
LaneB:The testis tissue lysate of Mouse
LaneC:MCF-7 whole cell lysate
LaneD:PC3 whole cell lysate

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Putative C->U-editing enzyme APOBEC-4; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4; APOBEC4; C1orf169

Immunogen: Synthetic peptide, corresponding to Human APOBEC4.

Conjugate: Unconjugated

Modification: Unmodified