Product Description

APRIL (S183) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1893-50, BS1893-100

Sizes: 50µl, 100µl

Swiss-Prot: O75888

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: A proliferation-inducing ligand (APRIL), also designated TNFSF13, is a Type II membrane protein that shares characteristics with other members of the tumor necrosis factor (TNF) cytokine family. APRIL is expressed in high levels in transformed cell lines and in human colon, thyroid, and lymphoid tumor tissues. APRIL is critically involved in the regulation of infections, inflammation, autoimmune diseases, and tissue homeostasis. APRIL is implicated in the regulation of tumor cell growth. The C-terminal extracellular domain has b jelly roll topography and is important in ligand trimerization. The binding of the ligand to its respective receptor induces oligomerization, initiating downstream signaling events. Intrinsic to oligomerization is the formation of the receptor binding site at the junction between neighboring subunits, creating a multivalent ligand.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: APRIL (S183) polyclonal antibody detects endogenous levels of APRIL protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of APRIL (S183) pAb at 1:500 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: Panc1 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of APRIL (S183) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 22 to 32 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; TRDL1; CD256; TNFSF13; APRIL; TALL2; ZTNF2; ORF Names: UNQ383/PRO715

Immunogen: Synthetic peptide, corresponding to amino acids 151-200 of Human APRIL.

Conjugate: Unconjugated

Modification: Unmodified