Product Description

APTX Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7755-50, BS7755-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7Z2E3

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100 | IP,1:20 - 1:50

Background: This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 40kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using APTX antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunofluorescence analysis of A549 cells using APTX antibody., Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody. Western blot was performed from the immunoprecipitate using APTX antibody at a dilution of 1:500.

Alternative Name: APTX; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT; aprataxin

Immunogen: Recombinant fusion protein of human APTX(NP_001182178.1).

Conjugate: Unconjugated

Modification: Unmodified