ARF4 (E106) Polyclonal Antibody-BS3065

ARF4 (E106) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3065-50, BS3065-100

Sizes: 50µl, 100µl

Swiss-Prot: P18085

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with Golgi membranes and the association of actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF1 gene maps to chromosome 1q42, contains 5 exons and 4 introns, and encodes a 181 amino acid protein.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ARF4 (E106) polyclonal antibody detects endogenous levels of ARF4 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ARF4 (E106) pAb at 1:500 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: L02 whole cell lysate (40ug)
Lane 4: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ARF4 (E106) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 21 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: ADP ribosylation factor 2; ADP ribosylation factor 4; ARF2;

Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human ARF4.

Conjugate: Unconjugated

Modification: Unmodified