Product Description

ARF6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90084-50, BS90084-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62330(Human) P62331(Mouse) P62332(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500

Background: The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of Actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF6 gene contains five exons and four introns, and encodes a 175 amino acid protein.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ARF6 polyclonal antibody detects endogenous levels of ARF6 protein.

Bioworld Molecular Weight: 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution., ICC staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ARF6, ADP ribosylation factor 6, ADP ribosylation factor protein 6, ADP-ribosylation factor 6, ARF6, ARF6, DKFZp564M0264, Small GTP binding protein, Small GTPase,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified