Product Description

Arfaptin 1 (K314) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3076-50, BS3076-100

Sizes: 50µl, 100µl

Swiss-Prot: P53367

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Arfaptin 1 (K314) polyclonal antibody detects endogenous levels of Arfaptin 1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Arfaptin 1 (K314) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: SP2/0 cell lysate
Lane 3: PC12 cell lysate
Lane 4: Rat muscle tissue lysate, Immunohistochemistry (IHC) analyzes of Arfaptin 1 (K314) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 40 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1; Arfaptin1

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human Arfaptin 1.

Conjugate: Unconjugated

Modification: Unmodified