Product Description

ARFGAP1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS8721-50, BS8721-100

Swiss-Prot: Q8N6T3

Host: Rabbit

ARFGAP1 Polyclonal Antibody-BS8721

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: ARFGAP1 (ADP-ribosylation factor GTPase activating protein 1), also known as ARF1 GAP, is a 406 amino acid protein that contains one ARF-GAP domain and localizes to the cytoplasm and the golgi apparatus. Functioning as a GTPase-activating protein (GAP), ARFGAP1 interacts with ARF1 and promotes hydrolysis of ARF1-bound GTP, an event that is required for both the dissociation of coat proteins from golgi structures and for the subsequent fusion of golgi vesicles with target compartments. When overexpressed, ARFGAP1, whose activity is inhibited by phosphatidylcholine and stimulated by phosphoinosides, can induce the redistribution of the entire golgi apparatus to the endoplasmic reticulum. Multiple isoforms of ARFGAP1 exist due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ARFGAP1 polyclonal antibody detects endogenous levels of ARFGAP1 protein.

Bioworld Molecular Weight: ~ 45kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody., Immunofluorescence analysis of A549 cell using ARFGAP1 antibody. Blue: DAPI for nuclear staining.

Alternative Name: ADP-ribosylation factor GTPase-activating protein 1,ARF GAP 1 ,ADP-ribosylation factor 1 GTPase-activating protein,ARF1 GAP,ARF1-directed GTPase-activating protein,ARFGAP1,ARF1GAP

Immunogen: Recombinant full length Human ARFGAP1.

Modification: Unmodified

Conjugate: Unconjugated