Product Description

Arginase 1 (ARG1) Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6971-50, BS6971-100

Swiss-Prot: P05089

Host: Rabbit

Arginase 1 (ARG1) Polyclonal Antibody-BS6971

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 40KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HepG2, using Arginase1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of human liver cancer cells using Arginase 1 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: ARG1; arginase-1

Immunogen: Recombinant fusion protein of human Arginase 1 (ARG1)(NP_000036.2).

Modification: Unmodified

Conjugate: Unconjugated