Product Description

ARHGEF7 (Q344) Polyclonal Antibody

Catalogue Numbers:BS3929-50, BS3929-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14155

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The serine/threonine kinase, p21 activated kinase (PAK), is a downstream effector of the small GTPases Cdc42 and Rac. PAK associates with Nck, the p85 and p110 subunits of PI3-kinase and PIX (PAK-interacting exchange factor) in a focal complex. The binding of PIX is necessary for the localization and activation of PAK in the Cdc42 to Rac signaling pathway, and this binding occurs through the high affinity of the N-terminal SH3 domain of PIX for a conserved proline rich PAK sequence. PIX exists as two isoforms, α and β, and both are highly expressed in heart, muscle and thymus tissues of human and rat. αPIX is phosphorylated via PDGF and EphB2 receptor signaling pathways or through association with PI3-kinase. The αPIX isoform predominantly acts as a guanine nucleotide exchange factor (GEF) on Rac, which may mediate lamellipodia formation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bioworld Molecular Weight: ~ 90 kDa

Specificity: ARHGEF7 (Q344) polyclonal antibody (Q344) polyclonal antibody detects endogenous levels of ARHGEF7 protein.

Note: For research use only, not for use in diagnostic procedure.
Alternative Name: PIXβ; Rho guanine nucleotide exchange factor 7; Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85; ARHGEF7; COOL1; KIAA0142; P85SPR; PAK3BP; PIXB; Nbla10314

Immunogen: Synthetic peptide, corresponding to amino acids 312-360 of Human ARHGEF7.

Conjugate: Unconjugated

Modification: Unmodified