Product Description

ARNT2 (M50) Polyclonal Antibody

Catalogue Numbers: BS2365-50, BS2365-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9HBZ2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: AhR, ARNT1, ARNT2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. BMAL1 (Brain and Muscle Arnt-like protein 1), also designated Arnt 3, TIC, JAP3 or MOP-3, has been shown to dimerize with Clock and bind to the promoter region of mPer1, suggesting that this protein plays a role in regulation of circadian oscillation in mammals.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ARNT2 (M50) polyclonal antibody detects endogenous levels of ARNT2 protein.

Extra Notes: Western blot (WB) analysis of Arnt2 (M50) pAb at 1:500 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:Hela whole cell lysate(40ug)
Lane5:H9C2 whole cell lysate(40ug)
Lane6:3T3-L1 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 79 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Aryl hydrocarbon receptor nuclear translocator 2; ARNT protein 2; Class E basic helix-loop-helix protein 1; bHLHe1; ARNT 2; BHLHE1; KIAA0307

Immunogen: Synthetic peptide, corresponding to amino acids 20-69 of Human ARNT2.

Conjugate: Unconjugated

Modification: Unmodified