Product Description

Aromatase Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90088-50, BS90088-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11511(Human) P28649(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:100
FC:1:50-1:100

Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased CYP19 activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7α-hydroxylase, CYP7A1, is the rate-limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Aromatase polyclonal antibody detects endogenous levels of Aromatase protein.

Bioworld Molecular Weight: 58 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution., ICC staining Aromatase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CYP19A1, ARO1, CYAR, CYP19, ARO, ARO1, Aromatase, CP19A, CPV1, CYAR, CYP19, Cyp19a1, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Cytochrome P450, family 19, subfamily A, polypeptide 1, Cytochrome P450, subfamily XIX (aromatization of androgens), Estrogen synthase, Estrogen synthetase, Flavoprotein linked monooxygenase, MGC104309, Microsomal monooxygenase, OTTHUMP00000162543, OTTHUMP00000198350, P 450AROM,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified