Arp3 Polyclonal Antibody-BS6115

Arp3 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6115-50, BS6115-100

Swiss-Prot: P61158

Host: Rabbit

Arp3 Polyclonal Antibody-BS6115

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100

Background: The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 47kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Arp3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit., Immunofluorescence analysis of HeLa cells using Arp3 antibody. Blue: DAPI for nuclear staining.

Alternative Name: ACTR3; ARP3

Immunogen: Recombinant fusion protein of human Arp3(NP_005712.1).

Modification: Unmodified

Conjugate: Unconjugated