Arp3 Polyclonal Antibody-BS90090

Arp3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90090-50, BS90090-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P61158(Human) Q99JY9(Mouse) Q4V7C7(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Arp3 polyclonal antibody detects endogenous levels of Arp3 protein.

Bioworld Molecular Weight: 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Arp3 on different lysates using anti-Arp3 antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Mouse placenta
Lane 3: Mouse thymus, ICC staining Arp3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ACTR3, ARP3, Actin like protein 3, Actin related protein 3, ACTR3, ARP3 actin related protein 3 homolog (yeast),

Immunogen: Recombinant protein of C-terminal human Arp3.

Conjugate: Unconjugated

Modification: Unmodified