Product Description

ARSA (R291) Polyclonal Antibody

Catalogue Numbers: BS3077-50, BS3077-100

Sizes: 50µl, 100µl

Alternative Name: Arylsulfatase A; ASA; Cerebroside-sulfatase; Arylsulfatase A component B; Arylsulfatase A component C; ARSA

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15289

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: ASA (arylsulfatase A), also known as Cerebroside-sulfatase, ARSA or MLD, is a 507 amino acid lysosomal protein that belongs to the sulfatase family. Functioning as a homodimer at a neutral pH and as a homooctamer at an acidic pH, ASA uses magnesium as a cofactor to catalyze the H2O-dependent hydrolysis of cerebroside 3-sulfate to cerebroside and sulfate. Defects in the gene encoding ASA are a cause of metachromatic leukodystrophy (MLD), an intralysosomal storage disease that is characterized by ataxias, dementia, seizures, spastic tetraparesis and, ultimately, death. Additionally, defects in ASA activity are associated with multiple sulfatase deficiency (MSD), a disorder that results in decreased activity of all known sulfatases and is generally characterized by metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ARSA (R291) polyclonal antibody detects endogenous levels of ARSA protein.

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ARSA (R291) pAb at 1:500 dilution
Lane 1: AML-12 whole cell lysate(40ug)
Lane 2: C6 whole cell lysate(40ug)
Lane 3: H1792 whole cell lysate(40ug)
Lane 4: HEK293T whole cell lysate(40ug)
Lane 5: PC3 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 260-310 of Human ARSA.

Conjugate: Unconjugated

Modification: Unmodified