Product Description

ASCL1 (E158) Polyclonal Antibody

Catalogue Numbers: BS3688-50, BS3688-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P50553

Host: Rabbit

Reactivity: Rat, Mouse, Human

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ASCL1 (E158) polyclonal antibody detects endogenous levels of ASCL1 protein.

Extra Notes: Western blot (WB) analysis of ASCL1 (E158) polyclonal antibody at 1:1000 dilution
Lane1:The brain tissue lysate of mouse(30ug)
Lane2:The brain tissue lysate of Rat(30ug)
Lane3:K562 whole cell lysate(30ug)
Lane4:BV2 whole cell lysate(30ug)
Lane5:A2780 cell membrane lysate(30ug)

Bioworld Molecular Weight: ~ 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Achaete-scute homolog 1; ASH-1; Hash1; Class A basic helix-loop-helix protein 46; bHLHa46

Immunogen: Synthetic peptide, corresponding to Human ASCL1.

Conjugate: Unconjugated

Modification: Unmodified