Product Description

ASH2L Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9923M-50, BS9923M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UBL3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:5000
IHC/ICC/IF: 1:200-1:500

Background: The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Alternative splicing results in a shorter isoform 2, designated ASH2L2, which is missing the first 94 amino acid residues found in ASH2L1. Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. ASH2L is highly expressed in fetal liver, testis and leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cells lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of ASH2L and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 69 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ASH2L Rabbit mAb at 1:1000 dilution
Lane 1: PC12 whole cell lysate
Lane 2: 293T whole cell lysate, Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody. Counter stained with hematoxylin., ICC staining ASH2L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: ASH 2;
Ash2 (absent small or homeotic) like;
Ash2 (absent, small, or homeotic) like (Drosophila);
ASH2;
ASH2 LIKE;
ASH2 like protein;
ASH2-like protein;
Ash2l;
ASH2L_HUMAN;
ASH2L1;
ASH2L2;
Bre 2;
Bre2;
Set1/Ash2 histone methyltransferase complex subunit ASH2;

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified