Product Description

Asparaginase (ASNase) Activity Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K909-M-48, E-BC-K909-M-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Category: Cell Metabolism Assays

Alternate Names: ASNase

Storage and Stability: This product can be stored at -20°C for 12 months with shading light.

Research Areas: Amino Acids and Proteins

Result Type: Enzyme activity

Detection Range: 1.27-182.52 U/L

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Tested Sample Types: Serum;Plasma

Assay Length: 40 min

Sensitivity: 1.27 U/L

Test Principle: Asparaginase (ASNase) is a proteolytic enzyme that was first discovered in guinea pigs and is not naturally produced in humans. Asparaginase has been widely used in the food industry, inhibiting the production of acrylamide carcinogens by hydrolyzing asparagine. Asparaginase is also used to treat various diseases due to its unique anti-tumor properties, such as acute lymphocytic leukemia, non-Hodgkin's lymphoma, leukemia meningitis, lung cancer and ovarian cancer and other tumor diseases.
The detection principle of this kit is as follows: asparaginase catalyzes the substrate to generate aspartic acid, and aspartic acid is hydrolyzed under the catalytic action of the enzyme. The amount of NADH consumed by the reaction is directly proportional to the activity of asparaginase. NADH is absorbed at a wavelength of 340 nm, and the activity of asparaginase can be calculated based on the change in absorbance value per unit time.

Inter CV: 7.1-8.2%

Intra CV: 2.2-4.7%

Research Use Only