Product Description

ATE1 Polyclonal Antibody

Catalogue Numbers: BS60103-50, BS60103-100

Sizes: 50µl, 100µl

Alternative Name: Arginyl-tRNA--protein transferase 1; Arginyltransferase 1; R-transferase 1; Arginine-tRNA--protein transferase 1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95260

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Arginyl-tRNA-protein transferase (ATE1), also designated arginyltransferase 1, belongs to the R-transferase family of proteins. In order for a protein to be degraded via the ubiquitin pathway, arginylation of the protein is required. ATE1 plays an important role in this process, as it is important for the posttranslational conjugation of arginine to the N-terminal aspartate-, glutamate- and possibly cystine-contaning substrates. ATE1 is a 518 amino acid protein. Alternative splicing results in two distinct isoforms. ATE1, which is found as a monomer, can localize to the cytoplasm and/or the nucleus.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATE1 polyclonal antibody detects endogenous levels of ATE1 protein.

Bioworld Molecular Weight: ~ 59 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATE1 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human ATE1.

Conjugate: Unconjugated

Modification: Unmodified