Product Description

ATF4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90099-50, BS90099-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P18848(Human) Q06507(Mouse) Q9ES19(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ATF4 polyclonal antibody detects endogenous levels of ATF4 protein.

Bioworld Molecular Weight: 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATF4 on different lysates using anti-ATF4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC-12, ICC staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: cAMP-dependent transcription factor ATF-4, CREB-2, cAMP-responsive element-binding protein 2, TaxREB67, ATF4, Activating transcription factor 4, ATF 4, ATF4, ATF4 protein, ATF4, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB 2, CREB-2, CREB2, Cyclic AMP dependent transcription factor ATF 4, Cyclic AMP response element binding protein 2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2, DNA binding protein TAXREB67, DNA-binding protein TAXREB67, Tax Responsive Enhancer Element B67, Tax-responsive enhancer element-binding protein 67, TaxREB67, TXREB,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified