Product Description

ATF6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90101-50, BS90101-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P18850(Human)

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles.

Specificity: ATF6 polyclonal antibody detects endogenous levels of ATF6 protein.

Bioworld Molecular Weight: 75 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATF6 on Hela cell lysate using anti-ATF6 antibody at 1/500 dilution., ICC staining ATF6 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Trx, ADF, SASP, TXN, TRDX, TRX, TRX1, Activating transcription factor 6 alpha, Activating transcription factor 6, ATF 6, ATF6 alpha, ATF6, ATF6-alpha, ATF6A, ATF6A, cAMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Cyclic AMP dependent transcription factor ATF 6 alpha, DKFZp686P2194, ESTM49, FLJ21663, Processed cyclic AMP dependent transcription factor ATF 6 alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha,

Immunogen: Recombinant protein within full sequence of human ATF6.

Conjugate: Unconjugated

Modification: Unmodified