ATG10 (P220) Polyclonal Antibody-AP0784

ATG10 (P220) Polyclonal Antibody

Catalogue Numbers: AP0784-50, AP0784-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H0Y0

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATG10 (P220) polyclonal antibody detects endogenous levels of ATG10 protein.

Extra Notes: Western blot (WB) analysis of ATG10 (P220) polyclonal antibody at 1:500 dilution
Lane1:Hct116 whole cell lysate(40ug)
Lane2:786-O whole cell lysate(40ug)
Lane3:SGC-7901 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Ubiquitin-like-conjugating enzyme ATG10; Autophagy-related protein 10; APG10-like; APG10L; PP12616

Immunogen: Synthetic peptide, corresponding to amino acids 200-245 of Human ATG1.

Conjugate: Unconjugated

Modification: Unmodified