Product Description

Atg12 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90103-50, BS90103-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O94817(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200

Background: Atg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5. Similarly in humans, Atg12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, Atg10 and ATP. The Atg12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two Atg12 isoforms exist.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Atg12 polyclonal antibody detects endogenous levels of Atg12 protein.

Bioworld Molecular Weight: 8/15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Human kidney tissue
Lane 3: 293T, Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Atg12 antibody. Counter stained with hematoxylin.

Alternative Name: APG12-like, ATG12, APG12, APG12L, APG12-like, APG12L, ATG12, ATG12 autophagy related 12 homolog (S. cerevisiae), ATG12 autophagy related 12 homolog, ATG12, Autophagy 12, Autophagy-related protein 12, FBR93, HAPG12, Ubiquitin-like protein ATG12,

Immunogen: Recombinant protein within human Atg12 aa 1-100.

Conjugate: Unconjugated

Modification: Unmodified