Product Description

ATG14 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-67885-60, E-AB-67885-120, E-AB-67885-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: ATG14

Target Synonym: ATG14; ATG14L; BARKOR; KIAA0831

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q6ZNE5

Background: Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity.

Concentration: 1 mg/mL

Immunogen: Recombinant fusion protein of human ATG14

Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IF 1:50-1:200

Calculated MW: 42kDa/55kDa

Observed MW: 65KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only