Product Description

ATG3 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67225-50, MB67225-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bμlk cytoplasmic contents. The molecμlar machinery of autophagy was largely discovered in yeast and referred to as autophagy-related genes (Atg). Formation of the autophagic vesicles involves two ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are essential for autophagy and widely conserved in eukaryotes. There are at least three Atg8 homologs in mammalian cells, GATE-16, GABARAP, and LC3, that are conjugated by lipids. Lipid conjugation of Atg8 and its mammalian homologs requires Atg3 (Apg3p/Aut1p in yeast), an ubiquitously expressed E2-like enzyme. Following C-terminal cleavage by the cysteine protease Atg4, the exposed glycine residue of Atg8 binds to the E1-like enzyme Atg7, is transferred to Atg3, and then conjugated to phophatidylethanolamine. Atg3-deficient mice die within 1 day after birth and are completely defective for the conjugation of Atg8 homlogs and autophagome formation.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB, IHC

Alternate Names: APG3, APG3L, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, APG3-like, hApg3, Protein PC3-96

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human ATG3. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of ATG3 protein.

Molecular Weight: ~ 40 kDa

Pathway: Autophagy Signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NT62

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only