Product Description

ATG4A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10586-50, MB10586-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q8WYN0

Host: Rabbit

Reactivity: Human

Applications: WB,IHC-P,IP

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATG4A in JurkatLysates using ATG4A antibody.

Bioworld Molecular Weight: Calculated MW: 45 kDa; Observed MW: 45 kDa

Specificity: IgG

Alternative Name: ATG4A; APG4A; AUTL2; Cysteine protease ATG4A; AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A

Immunogen: A synthetic peptide of human ATG4A

Conjugate: Unconjugated

Modification: Unmodified