Product Description

ATG4A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7735-50, BS7735-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WYN0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB 1:1000 - 1:2000

Background: Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg4a (ATG4 autophagy related 4 homolog A), also known as APG4A or AUTL2, is a 398 amino acid protein that localizes to the cytoplasm and belongs to the peptidase C54 family. Expressed in a variety of tissues, including brain, skeletal muscle and fetal liver, Atg4a functions as a cysteine protease that cleaves the C-terminal part of target proteins, such as GABARAP and MAP1LC3, and plays an essential role in autophagy. Atg4a exists as multiple alternatively spliced isoforms and is functionally inhibited by N-ethylmaleimide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATG4A polyclonal antibody detects endogenous levels of ATG4A protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of ATG4A pAb at 1:2000 dilution
Lane1:CT26 whole cell lysate(20ug)
Lane2:PC12 whole cell lysate(20ug)
Lane3:HCT116 whole cell lysate(20ug)
Lane4:PC3 whole cell lysate(20ug)

Alternative Name: Apg4a;
ATG4 autophagy related 4 homolog A (S. cerevisiae);
ATG4A;
ATG4A_HUMAN;
Atg4al;
AUT like 2 cysteine endopeptidase;
AUT-like 2 cysteine endopeptidase;
Autl2;
Autophagin 2;
Autophagin-2;
Autophagy related 4A cysteine peptidase;
Autophagy related cysteine endopeptidase 2;
Autophagy related protein 4 homolog A;
Autophagy-related cysteine endopeptidase 2;
Autophagy-related protein 4 homolog A;
AV169859;
Cysteine protease ATG4A;
hAPG4A;

Immunogen: Recombination protein , corresponding to amino acids 38-186 of Human ATG4A.

Conjugate: Unconjugated

Modification: Unmodified