Product Description

ATG4D (G30) Polyclonal Antibody

Catalogue Numbers: AP0777-50, AP0777-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q86TL0

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagyrelated gene (Atg) proteins. Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein that localizes to the cytoplasm and belongs to the C-54 family of cysteine proteases. Expressed predominately in skeletal muscle, but also present in testis, Atg4D functions as a cysteine protease that is required for autophagy and functions to specifically cleave the C-terminal region of target proteins, thereby allowing the target proteins to bind to autophagosomes. The enzymatic activity of Atg4D is inhibited by N-ethylmaleimide, a thiol reactive compound that is capable of modifying cystine residues in proteins and peptides.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATG4D(G30) polyclonal antibody detects endogenous levels of ATG4D protein.

Extra Notes: Western blot (WB) analysis of ATG4D (G30) polyclonal antibody at 1:1000 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:MCF-7 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 4; Autophagin-4; Autophagy-related protein 4 homolog D; HsAPG4D; Cysteine protease ATG4D, mitochondrial

Immunogen: Synthetic peptide, corresponding to Human ATG4D.

Conjugate: Unconjugated

Modification: Unmodified