Product Description

ATG4D Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70951-50, BS70951-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q86TL0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB 1:500 - 1:2000

Background: Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein that localizes to the cytoplasm and belongs to the C-54 family of cysteine proteases. Expressed predominately in skeletal muscle, but also present in testis, Atg4D functions as a cysteine protease that is required for autophagy and functions to specifically cleave the C-terminal region of target proteins, thereby allowing the target proteins to bind to autophagosomes. The enzymatic activity of Atg4D is inhibited by Nethylmaleimide, a thiol reactive compound that is capable of modifying cystine residues in proteins and peptides.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATG4D polyclonal antibody detects endogenous levels of ATG4D protein.

Bioworld Molecular Weight: ~ 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of ATG4D pAb at 1:1000 dilution
Lane1:The Lung tissue lysate of Mouse(40ug)
Lane2:The Lung tissue lysate of Rat(40ug)
Lane3:H1792 whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)

Alternative Name: APG4D; AUTL4; APG4-D;

Immunogen: Recombination protein, corresponding to amino acids 123-474 of Human ATG4D.

Conjugate: Unconjugated

Modification: Unmodified