Product Description

ATG5 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90105-50, BS90105-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H1Y0(Human) Q99J83(Mouse) Q3MQ06(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ATG5 polyclonal antibody detects endogenous levels of ATG5 protein.

Bioworld Molecular Weight: 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATG5 on different lysates using anti-ATG5 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Raji
Lane 2: Hela
Lane 3: PC12, ICC staining ATG5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ATG5, APG 5, APG 5L, APG5, APG5 autophagy 5 like, APG5 like, APG5-like, APG5L, Apoptosis specific protein, Apoptosis-specific protein, ASP, ATG 5, Atg5, ATG5 autophagy related 5 homolog, ATG5, Autophagy protein 5, Autophagy related 5, hAPG5, Homolog of S Cerevisiae autophagy 5, OTTHUMP00000040507,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified