Product Description

ATG7 Polyclonal Antibody

Product: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16858-50, BZ16858-100

Swiss-Prot: O95352

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF

All Applications: WB: 1/500-1/1000 IF: 1/50-1/200

Background: The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Calculated MW: 78 kDa; Observed MW: 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Atg7 in HepG2 lysates; mouse spleen lysates; rat kidney lysates using ATG7 antibody.

Alternative Name: hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L

Immunogen: A synthesized peptide derived from human Atg7 (Apg7)

Conjugate: Unconjugated

Modification: Unmodified