Product Description

ATGL/PNPLA2 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS7989-50, BS7989-100

Swiss-Prot: Q96AD5

Host: Rabbit

ATGL/PNPLA2 Polyclonal Antibody-BS7989

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 55kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunofluorescence analysis of U2OS cells using ATGL/PNPLA2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PNPLA2; 1110001C14Rik; ATGL; FP17548; PEDF-R; TTS-2.2; TTS2; iPLA2zeta

Immunogen: Recombinant fusion protein of human PNPLA2(NP_065109.1).

Modification: Unmodified

Conjugate: Unconjugated