Product Description

ATIC Monoclonal Antibody

Catalogue Numbers: MB66535-50, MB66535-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P31939

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis.Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR).Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction.Also catalyzes the cyclization of FAICAR to IMP.Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias.Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization.The de novo purine synthesis pathway includes 10 sequential steps, beginning with phosphoribosyl pyrophosphate and ending with inositol monophosphate (IMP), the first purin compound of the pathway.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of ATIC protein.

Extra Notes: Western blot analysis of ATIC expression in Jurkat (A), Hela (B), U2OS (C), mouse brain (D), rat liver (E) whole cell lysates.

Bioworld Molecular Weight: ~ 58 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: PURH; Bifunctional purine biosynthesis protein PURH

Immunogen: Purified recombinant human ATIC protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified