Product Description

ATM Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9893M-50, BS9893M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13315

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:5000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiec-tases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of ATM and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 370 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATM Rabbit mAb at 1:1000 dilution
Lane 1: CRC whole cell lysate, Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ATM antibody. Counter stained with hematoxylin., ICC staining ATM in Hela cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; ATM

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified