Product Description

ATP citrate lyase Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90107-50, BS90107-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P53396(Human) Q91V92(Mouse) P16638(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites. (Acetyl-CoA is not transported across subcellular membranes of plants.) These include: elongated fatty acids (used in seed oils, membrane phospholipids, the ceramide moiety of sphingolipids, cuticle, cutin, and suberin); flavonoids; malonic acid; acetylated phenolics, alkaloids, isoprenoids, anthocyanins, and sugars; and, mevalonate-derived isoprenoids (e.g., sesquiterpenes, sterols, brassinosteroids); malonyl and acyl-derivatives (d-amino acids, malonylated flavonoids, acylated, prenylated and malonated proteins). De novo fatty acid biosynthesis in plants is plastidic, thus ATP citrate lyase is not important for this pathway.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ATP citrate lyase polyclonal antibody detects endogenous levels of ATP citrate lyase protein.

Bioworld Molecular Weight: 122 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATP citrate lyase on mouse pancreas tissue (1) and NIH-3T3 cell (2) lysate using anti-ATP citrate lyase antibody at 1/200 dilution., ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ACL, ACLY, ACL, Acly, ACLY, ATP citrate (pro-S) lyase, ATP citrate lyase, ATP citrate synthase, ATP-citrate (pro-S-)-lyase, ATP-citrate synthase, ATPcitrate synthase, ATPCL, Citrate cleavage enzyme, CLATP, OTTHUMP00000164773,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified