Product Description

ATP1B2 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71372-50, BS71372-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14415

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 42kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ATP1B2 antibody at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s., Immunofluorescence analysis of C6 cells using ATP1B2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: ATP1B2; AMOG

Immunogen: Recombinant fusion protein of human ATP1B2(NP_001669.3).

Conjugate: Unconjugated

Modification: Unmodified