Product Description

ATP2A2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60240-50, BS60240-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16615

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The SERCAs mediate Ca2+ uptake into intracellular stores. SERCA-mediated Ca2+ uptake induces and maintains muscular relaxation. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue-dependent processing which is responsible for the generation of the SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle, and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells. SERCA2 plays an important part in regulating cardiac contractile function. SERCA3 is an isoform expressed in several cell types including platelets, lymphoid cells and mast cells. SERCA1, SERCA2 and SERCA3 all undergo alternative splicing.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATP2A2 polyclonal antibody detects endogenous levels of ATP2A2 protein.

Bioworld Molecular Weight: ~ 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of ATP2A2 polyclonal antibody at 1:500 dilution
Lane1:THP-1 whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:PC12 whole cell lysate

Alternative Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SR Ca(2+)-ATPase 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATP2B

Immunogen: A synthetic peptide corresponding to residues in Human ATP2A2.

Conjugate: Unconjugated

Modification: Unmodified